【文獻標題】Effects of dietary supplementation of inulin on rumen fermentation and bacterial microbiota, inflammatory response and growth performance in finishing beef steers fed high or low-concentrate diet
【作者】Ke Tian,Junhui Liu,Yawang Sun,et.al
【關鍵詞】Inulin,Rumen,Bacterial microbiota,Lipopolysaccharide,Beef steer
【出版期刊】《Animal Feed Science and Technology》
Plasma cytokines and APPs assay
The bovine-exclusive ELISA kit was used to measure the concentrations of plasma IL-1β, IL-6, IL-8 and TNF-α. The bovine ELISA kits were purchased from Shanghai Sinobest Biotechnology Co., Ltd. (Shanghai, China). APPs including LPS-binding protein (LBP),haptoglobin (Hp), serum amyloid A (SAA) and C-reactive protein (CRP) were determined using the bovine ELISA kits purchased from Shanghai Jinma Biotechnology Co., Ltd. (Shanghai, China). The determination was conducted according to the manufacturer’s instructions. All samples including the standards were tested in duplicate, and the optical density values were read on a microplate reader (Bio-Rad, xMarkTM, USA) at 450 nm.